Screening method for proline-rich proteins

Details Screening method for proline-rich proteins Screening method for proline-rich proteins

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2285480

A method for screening a pathogenic microorganism for a secreted or cell- surface protein potentially useful as a vaccine antigen involves the step of identifying proline-rich proteins, either as the amino acid level, or preferably at the DNA level by employing PCR. Suitable consensus sequences are described as (PXm)nP or (PPXq)nPP wherein: P is proline; X is any amino acid, m is from 1 to 5; q is 1 or 2; and n is 2 or more.

La présente invention concerne un procédé permettant de cribler un micro-organisme pathogène à la recherche d'une protéine sécrétée ou de surface pouvant être utilisée comme antigène vaccinal, lequel procédé consiste à identifier, au niveau de l'acide aminé ou, de préférence, au niveau de l'ADN par ACP, des protéines riches en proline. L'invention se rapporte aux séquences consensus appropriées suivantes: (PX¿m?)¿n?P ou (PPX¿q?)¿n?PP dans lesquelles P est la proline, X est l'acide aminé, m est compris entre 1 et 5; q est égal à 1 ou 2 et n est égal à 2 ou plus.

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