Screening molecules with anti-prion activity: kits, methods...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01)

Patent

CA 2502544

The invention concerns screening molecules with anti-prion activity. More particularly, it concerns kits for screening molecules with anti-prion activity characterized in that they comprise in combination a [<I>PSI+</I>], phenotype yeast, an antibiogram and an agent for purifying prions at sub-efficient doses, said yeast including the ade1-14 allele of the <I>ADE1</I> gene and an inactivated <I>ERG6</I> gene, the screening methods, and a family of molecules with anti-prion activity isolated by the inventive screen. The invention is applicable to anti-prion agents for producing medicines in particular for treating neurodegenerative diseases involving protein aggregates.

La présente invention est relative à du criblage de molécules à activité anti- prion. Elle vise plus particulièrement des kits de criblage de molécules à activité anti-prion, caractérisés en ce qu'ils comportent en combinaison une levure de phénotype [PSI+], un antibiogramme et un agent de curage des prions à des doses sub-efficaces, ladite levure présentant l'allèle adel-14 du gène ADE1 ainsi qu'un gène ERG6 inactivé, les méthodes de criblage, et une famille de molécules à activité anti-prion mie en évidence à l'aide du crible selon l'invention. Application des agents anti-prion pour fabriquer des médicaments en particulier pour le traitement de maladies neurodégénératives impliquant des agrégats protéiques.

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