C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/32 (2006.01) A01H 5/10 (2006.01) A01N 63/02 (2006.01) C07K 14/325 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2547933
The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
La présente invention concerne l~isolation et la caractérisation de séquences de nucléotides codant des protéines insecticides inédites secrétées dans l~espace extracellulaire à partir du Bacillus thuringiensis et de souches apparentées. Les protéines sont isolées de surnageants de culture du Bacillus thuringiensis et de souches apparentées et présentent une activité insecticide vis-à-vis d~insectes lépidoptères, notamment la pyrale du maïs (ECB), la noctuelle verdoyante du tabac (TBW) et la fausse-teigne des crucifères (DBM). Il est divulgué des protéines insecticides codées par des séquences de nucléotides qui s~hybrident, dans des conditions strictes, avec les séquences de nucléotides isolées et caractérisées. Il est divulgué des méthodes de construction et d~utilisation de cellules et de plantes transgéniques comprenant la séquence de nucléotides inédite de l~invention.
Donovan Judith
Donovan William
Engleman James
Malvar Thomas
Pitkin John
Mcfadden Fincham
Monsanto Technology Llc
LandOfFree
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