Secretion of antibodies without signal peptides from bacteria

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C07K 16/46 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01) C40B 40/08 (2006.01) C40B 40/10 (2006.01) C40B 50/06 (2006.01) C12N 15/13 (2006.01)

Patent

CA 2615776

The present invention is directed generally to compositions and methods for obtaining secretion of antibodies or antigen-binding antibody fragments from prokaryotes without the need for a signal peptide through making use of mutant host strains with altered secretory properties. In particular, the invention provides host cells and methods for obtaining secretion of antibodies or antigen-binding antibody fragments from bacteria without the need for a signal peptide and provides diverse libraries of antibody sequence resulting from such methods. The invention additionally provides diverse libraries.

La présente invention concerne d'une manière générale décomposition et des procédés permettant d'obtenir une sécrétion d'anticorps ou de fragments d'anticorps se liant l'antigène de procaryotes sans nécessiter la présence de peptide de signal par l'utilisation de souches hôtes mutantes avec des propriétés sécrétoires modifiées. Cette invention concerne en particulier des cellules hôtes et des procédés permettant d'obtenir une sécrétion d'antigène ou de fragments d'anticorps se liant à l'antigène de bactérie sans nécessiter la présence de peptide de signal et fournit diverses bibliothèques de séquences d'anticorps résultant de ces procédés. Cette invention concerne aussi diverses librairies.

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