Secretion of glycosylated proteins using a tissue...

Details Secretion of glycosylated proteins using a tissue... Secretion of glycosylated proteins using a tissue...

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/705 (2006.01) C07K 14/715 (2006.01) C12N 9/62 (2006.01) C12N 9/72 (2006.01) C12N 15/13 (2006.01) C12N 15/62 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2325022

DNA constructs, host cells and production methods are disclosed for the expression and recovery of polypeptides, especially those altered to have one or more glycosylation sites added or deleted. The DNA constructs, host cells and methods provided herein employ a DNA segment corresponding to a mammalian tissue plaminogen activator signal and/or pro-peptide.

On décrit des constructions d'ADN, des cellules hôtes et des procédés de production destinés à l'expression et à la récupération de polypeptides, en particulier les polypeptides que l'on a modifiés pour ajouter ou supprimer un ou plusieurs sites de glycosylation. Ces constructions d'ADN, cellules hôtes et procédés mettent en oeuvre un segment d'ADN correspondant à un signal et/ou pro-peptide de l'activateur tissulaire du plasminogène chez le mammifère.

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