Secretory expression in eukaryotes

Details Secretory expression in eukaryotes Secretory expression in eukaryotes

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/81 (2006.01) C07K 14/485 (2006.01) C07K 19/00 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1341297

Methods and compositions are provided for producing polypeptide sequences in high yield by employing DNA constructs, wherein the DNA sequence encoding for the polypeptide of interest is preceded by a leader sequence and processing sequence for secreting and processing said polypeptide. In this manner, the mature polypeptide of interest may be isolated from the nutrient medium substantially free of major amounts of other proteins and cellular debris.

441501

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