Selectable marker for development of vectors and...

C - Chemistry – Metallurgy – 12 – N

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195/1.18, 195/1.

C12N 15/84 (2006.01) A01H 4/00 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/82 (2006.01)

Patent

CA 1340766

The present invention discloses functional and selectable micro-Ti plasmids. The hygromycin phosphotransferase (aphIV) gene from Escherichia coli was inserted between the 5' promoter and associated amino terminal region-encoding sequence of an octopine synthetase gene and the 3' terminator signal sequence of a nopaline synthetase gene. These constructs were assembled between T-DNA border fragments in a broad-host-range vector and used to create antibiotic-resistant plant cells.

497740

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