Selection and isolation of living cells using rna-binding...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) A01K 67/027 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 5/00 (2006.01) C12N 15/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2496821

The invention is directed to reliable and efficient detection of mRNAs as well as other RNAs in living cells and its use to identify and, if desired, separate cells based on their desired characteristics. Such methods greatly simplify and reduce the time necessary to carry out previously-known procedures, and offers new approaches as well, such as selecting cells that generate a particular protein or antisense oligonucleotide, generating cell lines that express multiple proteins, generating cell lines with knock-out of one or more protein, and others.

La présente invention concerne un procédé de détection fiable et efficace de l'ARNm et d'autres ARN dans des cellules vivantes et son utilisation pour identifier et, si désiré, séparer les cellules sur la base de caractéristiques souhaitées. Les procédés de l'invention permettent de simplifier dans une large mesure les procédures connues jusqu'à présent et de réduire le temps qui était nécessaire à leur mise en oeuvre, et offrent de nouvelles approches permettant, par exemple, de choisir des cellules produisant un oligonucléotide antisens ou une protéine particulière, de produire des lignées cellulaires exprimant des protéines multiples, de produire des lignées cellulaires avec invalidation d'une ou plusieurs protéines, et autres.

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