C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/80 (2006.01) C12N 1/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/01 (2006.01) C12N 15/09 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/65 (2006.01) C12N 15/70 (2006.01) C12N 15/73 (2006.01) C12N 15/74 (2006.01) C12N 15/75 (2006.01) C12N 15/81 (2006.01)
Patent
CA 2128589
The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
Selten Gerardus Cornelis Maria
Swinkels Bart Willem
Van Gorcom Robertus Franciscus Maria
Dsm Ip Assets B.v.
Gist-Brocades N.v.
Osler Hoskin & Harcourt Llp
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