C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01)
Patent
CA 2672710
The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.
L'invention concerne un procédé de production d'une cellule végétale transformée. Le procédé concerne, plus particulièrement, la transformation d'une cellule végétale à l'aide d'une cassette de transformation ciblée pour des plastes végétaux et comprenant un gène de sélection, par exemple l'isopentényle transférase (IPT), et un transgène. Suite à la sélection des plastes transformés, l'expression d'une recombinase est induite dans la cellule végétale, menant à l'excision du gène de sélection du plaste et à l'expression du transgène dans le plaste. L'invention concerne également des cellules et des plantes comprenant la cassette de transformation.
Chua Nam-Hai
Moller Simon Geir
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Plastid As
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