Selection method for cell internalizing nucleic acids

Details Selection method for cell internalizing nucleic acids Selection method for cell internalizing nucleic acids

C - Chemistry, Metallurgy

40

B

C40B 10/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/44 (2006.01) C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C40B 30/00 (2006.01)

Patent

CA 2683602

The present invention includes compositions and methods for contacting one or more cells with a random RNA-containing library, treating the contacted cells and with a denaturing agent or digestion with one or more nucleases, and extracting from the cells one or more internalized nucleic acids resistant to the nucleases or denaturants.

La présente invention concerne des compositions et des procédés consistant à mettre une ou de plusieurs cellules en contact avec une bibliothèque contenant des ARN aléatoires, traiter les cellules mises en contact avec un agent de dénaturation ou les digérer avec une ou plusieurs nucléases et extraire des cellules un ou plusieurs acides nucléiques internalisés résistants aux nucléases ou aux agents de dénaturation.

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