Selection method ii

Details Selection method ii Selection method ii

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01)

Patent

CA 2727560

The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.

Linvention concerne un procédé de fabrication dune cellule de plante transformée. Plus particulièrement, le procédé comprend la transformation dune cellule de plante avec une cassette de transformation qui cible les plastes des plantes et qui comprend un gène de sélection, par exemple isopentényle transférase (IPT), et un transgène. Après la sélection des plastes transformés, lexpression dune recombinase est induite dans la cellule de plante, ce qui conduit à lexcision du gène de sélection du plaste et à lexpression du transgène dans le plaste. Linvention concerne également des cellules et des plantes qui comprennent la cassette de transformation.

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