C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 21/00 (2006.01) C12N 15/11 (2006.01) C12N 15/52 (2006.01) C12N 15/74 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2436519
The invention provides improved library selection procedures for nucleic acids which allow the rapid determination of accessible target sites throughout relatively long target RNAs. This invention provides an improved method of screening a library of nucleic acids to identify cleavage sites of a target RNA. The steps of the screening comprise generating the library of nucleic acids, wherein each nucleic acid comprises a catalytic core flanked by random nucleotides; adding said target RNA to the library of nucleic acids; and isolating nucleic acids that cleave said target RNA. The nucleic acids selected by the methods described herein are also provided in the invention.
L'invention concerne des méthodes perfectionnées de sélection de bibliothèques d'acides nucléiques permettant de déterminer rapidement des sites cibles accessibles dans des ARNs cibles relativement longs. L'invention concerne également une méthode perfectionnée de criblage d'une bibliothèque d'acides nucléiques visant à identifier les sites de clivage d'un ARN cible. Les étapes du criblage consistent à générer une bibliothèque d'acides nucléiques, dans laquelle chaque acide nucléique comprend un noyau catalytique flanqué de nucléotides aléatoires; à ajouter ledit ARN cible à la bibliothèque d'acides nucléiques; et à isoler des acides nucléiques coupant ledit ARN cible. L'invention concerne également les acides nucléiques sélectionnés par lesdites méthodes.
Clawson Gary A.
Pan Wei-Hua
Sim & Mcburney
The Penn State Research Foundation
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