Selection, propagation and use of mosaic aneuploid stem cells

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 5/071 (2010.01) C12N 5/074 (2010.01) A61K 35/12 (2006.01) C12N 5/02 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2665002

The distribution of cell karyotypes within a population of cells can determine the phenotype and ability of stem cells to differentiate into desired cell types, to function normally, as well as represent risk for adverse events like cancer. Therefore, determination of the aneuploid mosaic status of a cell population is useful in identifying and/or maintaining desirable traits and eliminating undesirable traits in stem cells, and for defining them at the level of their chromosomal complement.

La répartition des karyotypes au sein d'une population de cellules peut déterminer le phénotype de cellules souches et leur aptitude à se différencier en types de cellule recherchés, et donc à fonctionner normalement ou bien représenter un risque de type cancer notamment. C'est pourquoi il est utile de déterminer l'état mosaïque aneuploïde d'une population cellulaire pour l'identification et/ou le maintien de traits souhaitables et pour l'élimination de traits indésirables dans des cellules souches, ainsi que pour la définition de ces traits au niveau de leur complément chromosomique.

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