Selection using opine synthase genes

Details Selection using opine synthase genes Selection using opine synthase genes

C - Chemistry, Metallurgy

12

N

150/16.5, 150/8.

C12N 15/00 (2006.01) A01H 1/00 (2006.01) C07K 14/325 (2006.01) C12N 5/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 1251386

Abstract of the Disclosure A method is described for selecting non-tumorous transformed plant cells expressing a gene coding for an opine synthase and containing a plant expressible heterologous gene from a mixture containing transformed plant cells and untransformed plant cells. The method involves plating the mixture on a growth medium containing an amino acid analog toxic to normal cells but metabolized by a plant cell expressing the opine synthase encoded by the gene, growing the mixture on the growth medium and selecting colonies of plant cells exhibiting greater growth rates.

560626

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