Selective 5' ligation tagging of rna

Details Selective 5' ligation tagging of rna Selective 5' ligation tagging of rna

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/02 (2006.01) C12N 15/11 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2723265

The present invention provides novel compositions, kits and methods employing RNA 5' polyphosphatases, RNA 5' monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5' ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5' ends. The 5'tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

l'invention concerne des compositions, des trousses et des procédés nouveaux utilisant des ARN 5' polyphosphatases, des ARN 5' monophosphatases, des enzymes coiffants, des enzymes décoiffants, des pyrophosphatases d'acide nucléique et des ligases d'ARN, ainsi que d'autres enzymes, pour le marquage sélectif par ligature en 5' des classes de molécules d'ARN recherchées qui diffèrent par rapport à des fractions chimiques particulières sur leurs extrémités 5'. Les molécules d'ARN marquées en 5' peuvent être utilisées pour la synthèse d'un ADNc premier brin, double brin, et d'ARN sens ou antisens pour diverses utilisations.

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