G - Physics – 01 – N
Patent
G - Physics
01
N
167/43
G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2024051
X-7975 ABSTRACT The invention is a method for selectively isolating cells which secrete higher levels of monoclonal antibody than did the parental culture from which they were derived. The method is based on the membrane characteristics of the cell and requires the use of a cell sorter. At present, the invention's best mode utilizes fluorescent probes in conjunction with a fluorescence activated cell sorter. The method is based on the correlation of a high level of cell surface immunoglobulin with high monoclonal antibody secretion rates. The invention provides a predictable and rapid process for selecting high monoclonal antibody producing cells.
Maciak Ronald S.
Marder Philip
Eli Lilly And Company
Gowling Lafleur Henderson Llp
Maciak Ronald S.
Marder Philip
LandOfFree
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