Selective expansion of target cell populations

A - Human Necessities – 61 – K

Patent

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Details

A61K 35/14 (2006.01) A61K 35/28 (2006.01) C12M 3/00 (2006.01) C12P 1/00 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2221433

The invention provides a method of selective expansion of a predetermined targetpopulation of cells, including (a) introducing a starting sample of cells into a growth medium; (b) causing cells of the predetermined target cell population to divide; and (c) contacting the starting cells with a selection element, comprising a plurality of selective binding molecules with specific affinity either for target cells or for non-target cells to select cells of said predetermined target population from other cells in the growth medium. The invention also provides systems for selective expansion and methods of treating patients with cell populations and products.

L'invention fournit une méthode d'expansion sélective d'une population cible prédéterminée de cellules, comportant les étapes suivantes a) introduction d'un échantillon de départ de cellules dans un milieu de croissance, b) division des cellules de la population cible de cellules et c) mise en contact des cellules de départ avec un élément de sélection, comprenant une série de molécules de liaison sélective ayant une affinité spécifique soit pour les cellules cibles soit pour des cellules non visées afin de choisir les cellules de la population cible prédéterminée en question parmi les autres cellules dans le milieu de croissance. L'invention fournit aussi des systèmes pour l'expansion sélective, ainsi que des méthodes de traitement de patients avec des populations et des produits de cellules.

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