Selective incorporation of 5-hyroxytryptophan into proteins...

Details Selective incorporation of 5-hyroxytryptophan into proteins... Selective incorporation of 5-hyroxytryptophan into proteins...

A - Human Necessities

61

K

A61K 38/43 (2006.01) C07H 21/02 (2006.01) C07K 1/00 (2006.01) C12N 9/00 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2549830

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

La présente invention a trait à des procédés et des compositions pour l'incorporation d'un acide aminé non naturel dans un peptide au moyen d'une paire orthogonale d'aminoacyl ARNt synthétase/ARNt. En particulier, une paire orthogonale est fournie pour l'incorporation de 5-hydroxy-L-tryptophane dans une position codée par une mutation opale.

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