Selective media for recovery and enumeration of campylobacters

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01)

Patent

CA 2376053

A composition for recovery and enumeration of <i>Campylobacter</i> species that includes a selective <i>Campylobacter</i> medium plus an indicator, 2,3,5- triphenyltetrazolium chloride. Improved selective media including the indicator, contains a nutrient medium with an energy source, agar, a reducing agent, and selective agents. The selective agents are a mixture of agents selected from cycloheximide, cefoperazone, vancomycin, trimethoprim, polymyxin B; rifampicin, amphotericin, and nystatin. The salts of these agents are also useful.

L'invention concerne une composition destinée à la récupération et au dénombrement d'espèces dénommées <i>Campylobacter</i>. Cette composition contient un milieu sélectif vis-à-vis de <i>Campylobacter</i> associé à un indicateur, le chlorure de 2,3,5-triphényltétrazolium. Les milieux sélectifs perfectionnés contenant l'indicateur, comportent un milieu nutritif associé à une source d'énergie, l'agar-agar, à un agent réducteur et à des agents sélectifs. Lesdits agents sélectifs sont un mélange d'agents sélectionnés parmi le cycloheximide, la céfopérazone, la vancomycine, le triméthoprime, la polymyxine B, la rifampicine, l'amphotéricine et la nystatine. Les sels de ces agents peuvent également être utilisés.

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