Selective recognition of solutes in chromatographic media by...

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B01J 20/26 (2006.01) B01D 15/08 (2006.01) B01D 15/38 (2006.01) B01J 20/291 (2006.01) G01N 30/02 (2006.01)

Patent

CA 2199233

Chromatographic media that demonstrate an artificially created recognition of any preselected molecular species with only an affinity-type adsorption are formed by polymerization from solutions of non-ionizable monomers with a quantity of the species contained in the solution, followed by removal of the species by conventional washing. The media are of particular utility and interest when used to achieve affinity binding of proteins. Uses of the media include chromatography and the general isolation and removal of the adsorbed species.

Milieux chromatographiques possédant une capacité de reconnaissance créée artificiellement pour toute espèce moléculaire présélectionnée, avec seulement une adsorption de type affinité; ces milieux sont obtenus par polymérisation à partir de solutions de monomères non ionisables avec une certaine quantité de l'espèce présente dans les solutions, suivie de l'élimination de l'espèce par lavage classique. Ces milieux sont particulièrement utiles et intéressants lorsqu'ils servent à la fixation des protéines par affinité. Parmi les applications pour ces milieux, on peut citer la chromatographie ainsi que l'isolation et l'élimination générales de l'espèce adsorbée.

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