Selective template deletion method

Details Selective template deletion method Selective template deletion method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2195842

A novel method for inactivating undesirable members in a nucleic acid sample which comprises undesirable and desirable members of a nucleic acid family. The method includes the step of adding a blocker to the sample which selectively associates with the undesirable members such that they are unable to participate in any further processing of the sample. The method advantageously provides a means whereby desirable members of the nucleic acid family can be processed, i.e. identified, amplified and/or isolated, without interference from undesirable sequence-related members.

Nouvelle méthode pour inactiver des constituants indésirables d'un échantillon d'acides nucléiques, lequel renferme des membres souhaitables et non souhaitables d'une famille d'acides nucléiques. La méthode comprend une étape d'addition d'un agent de blocage à l'échantillon, qui s'associe sélectivement avec les membres non souhaitables, de telle façon qu'ils ne puissent plus participer à un quelconque traitement ultérieur de l'échantillon. La méthode représente un moyen utile permettant de traiter, c.-à-d. caractériser, amplifier et (ou) isoler les membres souhaitables de la famille d'acides nucléiques, sans interférence par des membres non souhaitables liés par une séquence.

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