Selective terminal tagging of nucleic acids

Details Selective terminal tagging of nucleic acids Selective terminal tagging of nucleic acids

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2639819

Methods are provided for adding a terminal sequence tag to nucleic acid molecules for use in RNA or DNA amplification. The tag introduced may be used as a primer binding site for subsequent amplification of the DNA molecule and/or sequencing of the DNA molecule and therefore provides means for identification and cloning of the 5'-end or the complete sequence of mRNAs.

La présente invention se rapporte à des procédés permettant d'ajouter un marqueur de séquence terminale à des molécules d'acide nucléique aux fins d'utilisation pour l'amplification d'ARN ou d'ADN. Le marqueur introduit peut servir de site de liaison d'amorce pour l'amplification ultérieure de la molécule d'ADN et/ou le séquençage de cette dernière, et contribue donc à l'identification et au clonage de l'extrémité 5' ou de la séquence complète d'ARNm.

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