Self-addressable self-assembling microelectronic systems and...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C07H 21/00 (2006.01) C07K 1/04 (2006.01) C12M 1/42 (2006.01) C12N 15/10 (2006.01) C12P 1/00 (2006.01) C12P 21/00 (2006.01) C12Q 1/68 (2006.01) C40B 60/00 (2006.01) G11C 13/02 (2006.01) H01L 29/78 (2006.01)

Patent

CA 2169852

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Un dispositif microélectronique à auto-adressage et auto-assemblage est conçu et fabriqué pour effectuer et maîtriser activement des réactions de biologie moléculaire multiplex et multi-étapes à formats microscopiques. Ces réactions incluent des hybridations d'acides nucléiques, des réactions anticorps/antigènes, des diagnostics et des synthèses de biopolymères. Ce dispositif peut être fabriqué aussi bien avec des techniques de microlithographie que de micro-usinage. Ce dispositif peut maîtriser le tranport et la fixation d'entités à liaisons spécifiques en des micro-sites spécifiques. Ces entités à liaisons spécifiques incluent des molécules de biologie moléculaire telles que des acides nucléiques et des polypeptides. Ce dispositif peut ensuite maîtriser le transport et la réaction d'analytes ou de réactants sur les micro-sites adressés spécifiques. Il peut concentrer de tels analytes et réactants, éliminer des molécules à liaison non spécifique, fournir une régulation stringente pour des réactions d'hybridation d'ADN, et améliorer la détection d'analytes. Ce dispositif peut aussi être reproduit par des moyens électroniques.

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