Self-assembling split-fluorescent protein systems

Details Self-assembling split-fluorescent protein systems Self-assembling split-fluorescent protein systems

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12N 15/12 (2006.01) C12N 15/63 (2006.01) C12N 15/65 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2585231

The invention provides a protein labeling and detection system based on self- complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self- complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

L'invention concerne un système de marquage et de détection de protéines reposant sur des fragments qui s'auto-complémentent de protéines fluorescentes et chromophores. Le système de l'invention peut être illustré par diverses combinaisons de fragments auto-complémenteurs dérivés de la Protéine verte fluorescente (GFP) d'Aequora victoria, qui s'utilisent pour la détection et la quantification de la solubilité de la protéine dans des essais multiples, tant in vitro qu'in vivo.

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