Self-containing lactobacillus strain comprising a thya...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) A61K 35/74 (2006.01) A61K 38/20 (2006.01) A61P 29/00 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2506031

The invention relates to a recombinant Lactobacillus strain, with limited growth and viability in the environment. More particularly, it relates to a recombinant Lactobacillus that can only survive in a medium, where well- defined medium compounds, preferably thymidine or thymine, are present. A preferred embodiment is a Lactobacillus that may only survive in a host organism, where said medium compounds are present, but cannot survive outside the host organism in absence of said medium compounds. Moreover, said Lactobacillus strain can be transformed with prophylactic and/or therapeutic molecules and can, as such, be used to treat diseases such as, but not limited to, inflammatory bowel diseases.

La présente invention concerne une souche de lactobacillus recombinant, dont les capacités de croissance et de survie sont limitées en environnement ordinaire. L'invention concerne plus particulièrement un lactobacillus ne pouvant survivre que dans un milieu contenant des composés bien définis, de préférence la thymidine ou la thymine. Un mode de réalisation préféré concerne un lactobacillus qui ne peut survivre que dans un organisme hôte, dans lequel sont présents les composés du milieu, mais ne peut pas survivre à l'extérieur de l'organisme hôte en l'absence des composés du milieu. En outre, cette souche de lactobacillus peut être transformée au moyen de molécules prophylactique et/ou thérapeutique, et peut, en tant que telle, servir au traitement de maladies telles que notamment la maladie inflammatoire de l'intestin.

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