Self-replicating vector lacking an antibiotic-resistance gene

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/70 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2730741

The subject matter of the present invention is a novel vector and the use thereof for producing a heterologous protein or a gene of interest, that can be used, for example, in the context of an immunization or gene therapy programme and concerns in particular a self-replicating vector lacking an antibiotic- resistance gene, comprising a sequence encoding the ccdA protein functionally linked to a first promoter, the sequence of the Cer locus and a heterologous sequence, functionally linked to a second promoter.

La présente invention a pour objet un nouveau vecteur et son utilisation pour la production d'une protéine hétérologue ou d'un gène d'intérêt, utilisable par exemple dans le cadre d'un programme d'immunisation ou de thérapie génique et concerne en particulier vecteur auto réplicatif dépourvu de gène de résistance à un antibiotique comprenant une séquence codant pour la protéine ccdA liée de façon fonctionnelle à un premier promoteur, la séquence du locus Cer et une séquence hétérologue, liée de façon fonctionnelle à un deuxième promoteur.

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