C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 23/00 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2172248
This invention relates to an improved process for detecting and quantifying a de- sired nucleic acid sequence. This process involves synthesizing single stranded RNA, single stranded DNA, double-stranded DNA followed by detection using an electrochemi- luminescent labeled binding species. The fig- ure illustrates an electrochemiluminescent la- beled nucleotide for polymerase incorporation in nucleic acid.
Cette invention se rapporte à un procédé amélioré pour détecter et quantifier une séquence d'acides nucléiques désirée. Ce procédé consiste à synthétiser un ARN simple brin, un ADN simple brin et un ADN double brin, puis à effectuer l'opération de détection en utilisant une espèce de liaison marquée électrochimioluminescente. La figure illustre un nucléotide marqué électrochimioluminescent (ECL) pour l'incorporation de polymérase dans l'acide nucléique.
Kenten John
Smith Rodger
Bioveris Corporation
Gowling Lafleur Henderson Llp
Igen Inc.
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