Seminal vesicle tissue-specific promoters and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) A01K 67/027 (2006.01) C07K 14/47 (2006.01) C12N 5/10 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2471160

The present invention relates to genetically modified animals for the production of recombinant proteins. Particularly, pig's seminal vesicle and sexual accessory glands are genetically transformed to recombinantly produce such protein of interest in the semen of sexually mature pigs. Even complex proteins can be produced in large quantities and purified. The present invention also relates to mammal seminal gland tissue-specific promoters that can be used to make gene constructs including a protein-coding sequence not natively associated with the promoters or a sufficient portion of the promoter such that the portion actuates the preferential expression of the protein- coding sequence in the seminal vesicle and/or accessory tissues of mammals.

L'invention concerne des animaux génétiquement modifiés en vue de la production de protéines de recombinaison. Plus précisément, la vésicule séminale et les glandes sexuelles accessoires du porc sont génétiquement transformées en vue de produire par recombinaison une telle protéine d'intérêt dans le sperme de porcs sexuellement adulte. On peut même produire et purifier en grandes quantités des protéines complexes. L'invention concerne également des promoteurs spécifiques au tissu des glandes séminales de mammifères que l'on peut utiliser pour fabriquer des gènes chimères comprenant une séquence codant pour une protéine qui n'est pas associée de façon naturelle aux promoteurs ou une partie suffisante du promoteur de sorte que ladite partie déclenche l'expression préférentielle de la séquence codant pour la protéine dans la vésicule séminale et les tissus accessoires de mammifères.

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