C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) C08B 30/04 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2280210
A method of inhibiting gene expression is described. The method, which affects enzymatic activity in a plant, comprises expressing in a plant (or a cell, a tissue or an organ thereof) a nucleotide sequence wherein the nucleotide sequence codes, partially or completely, for a class A SBE intron in a sense orientation; and wherein the nucleotide sequence does not contain a sequence that is sense to an exon sequence normally associated with the intron.
L'invention concerne une méthode d'inhibition de l'expression d'un gène. Cette méthode, laquelle affecte l'activité enzymatique dans un végétal, consiste à exprimer dans un végétal (ou une cellule, un tissu ou bien un organe de celui-ci) une séquence nucléotidique codant partiellement ou totalement pour un intron de l'enzyme ramifiante de l'amidon de classe A dans une orientation sens, cette séquence nucléotidique ne contenant pas de séquence qui soit sens vis-à-vis d'une séquence d'exon normalement associée avec l'intron.
Danisco A/s
Gowling Lafleur Henderson Llp
LandOfFree
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