Sensing system for specific substance and molecule detection

G - Physics – 01 – N

Patent

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Details

G01N 33/543 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2286134

A system for detecting a specific substance or analyte of interest is provided that includes one or more sensing units and an instrument for analyzing the sensing units. Each sensing unit preferably includes a substrate (108), an attachment layer and at least one capture layer that includes a ligand layer. In one embodiment, the attachment layer is tripartite and includes a lower binding surface (118) held to the substrate and an upper binding surface (120) that holds the ligand layer (116), together with an insulating layer (124) disposed between these two surfaces. The lower binding surface provides a durable and stable attachment to the substrate. The upper binding surface holds the ligand layer and does not jeopardize the integrity or viability thereof. The insulating layer prevents any unwanted interaction between the lower and upper binding surfaces. Each sensing unit is supported on a test piece received by the instrument.

Cette invention se rapporte à un système conçu pour la détection d'une substance ou d'un analyte spécifique d'intérêt, qui comporte une ou plusieurs unités de détection et un outil d'analyse des unités de détection. Chaque unité de détection comporte de préférence un substrat (108), une couche de fixation et au moins une couche de capture comprenant une couche ligand. Selon une réalisation, la couche de fixation est tripartite et comprend une surface de liaison inférieure (118) attachée au substrat et une surface de liaison supérieure (120) qui incorpore la couche ligand (116), ces deux surfaces étant séparées par une couche isolante (124). La couche de liaison inférieure assure une fixation durable et stable au substrat. La surface de liaison supérieure incorpore la couche ligand et ne met pas en péril l'intégrité ou la viabilité de cette couche. La couche isolante empêche toute interaction entre les surfaces de liaison inférieure et supérieure. Chaque unité de détection est supportée par une éprouvette logée dans l'instrument.

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