Separating unmodified hemoglobin from cross-linked hemoglobin

Details Separating unmodified hemoglobin from cross-linked hemoglobin Separating unmodified hemoglobin from cross-linked hemoglobin

C - Chemistry, Metallurgy

07

K

C07K 14/805 (2006.01) A61K 38/42 (2006.01)

Patent

CA 2222679

A method for separating unmodified hemoglobin from cross-linked hemoglobin in a hemoglobin solution. The method involves contacting the hemoglobin solution with at least one dissociating agent to form a dissociation solution wherein unmodified tetrameric hemoglobin is dissociated to form hemoglobin dimers. The hemoglobin dimers are then separated from the dissociation solution, while retaining the cross-linked hemoglobin in the dissociation solution.

Procédé de séparation d'hémoglobine non modifiée et d'hémoglobine réticulée dans une solution d'hémoglobine, consistant à mettre la solution d'hémoglobine en contact avec au moins un agent de dissociation pour former une solution de dissociation dans laquelle l'hémoglobine tétramère non modifiée est dissociée sous forme de dimères d'hémoglobine. Ces dimères d'hémoglobine sont ensuite séparés de la solution de dissociation, tandis que l'hémoglobine réticulée est retenue dans ladite solution.

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