B - Operations – Transporting – 01 – D
Patent
B - Operations, Transporting
01
D
B01D 21/26 (2006.01) G01N 1/28 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2518555
The present invention provides for methods for proteome fractionation through the separation and accumulation of subcellular organelles from a biological sample such that the subcellular organelles are highly enriched, substantially pure, and whose structural integrity and functions are well-preserved. The methods of the invention provide a manner by which to reduce the complexity of the proteome and facilitate the detection and isolation of difficult-to-study proteins, such as low-abundance proteins. The methods of the present invention for pre-fractionating proteomes of biological samples by parallel separation and isolation of subcellular organelles from the biological samples using continuous-flow ultracentrifugation are also easily and effectively scalable through adjustment to ultracentrifugation parameters, such as, for example, rotor speed, rotor size, rotor geometry.
La présente invention concerne des méthodes de fractionnement du protéome par séparation et accumulation d'organites subcellulaires provenant d'un échantillon biologique de telle sorte que ces organites subcelluliares soient fortement enrichis, sensiblement purs et que leur intégrité structurelle et leurs fonctions soient bien préservées. Les méthodes de l'invention permettent de réduire la complexité du protéome et de détecter et d'isoler plus facilement des protéines difficiles à étudier, telles que des protéines de faible abondance. Les méthodes de l'invention concernant le pré-fractionnement du protéome tiré d'échantillons biologiques à- séparation parallèle et isolation d'organites subcellulaires par ultracentrigugation en flux continu sont par ailleurs facilement et efficacement extensibles par ajustement des paramètres du rotor de centrifugation tels que vitesse, taille ou géométrie.
Alfa Wassermann Inc.
Smart & Biggar
LandOfFree
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