C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/42 (2006.01) C07K 1/14 (2006.01) C07K 1/22 (2006.01) C07K 19/00 (2006.01) C12N 9/16 (2006.01) C12N 9/24 (2006.01) C12P 19/02 (2006.01)
Patent
CA 2226785
Novel methods for separating and/or purifying biological compositions are provided. The biological compositions comprise a polysaccharide binding peptide and can be synthesized in whole or in part or prepared by recombinant DNA technology. In the method, a solution containing the biological composition is mixed with a phase-forming oligosaccharide to which the binding peptide binds and phase separation is induced. The phase containing the biological composition is then isolated. Phase separation is induced using a phase separation inducing agent or, when the oligosaccharide polymer is a thermoseparating polymer, by increasing the temperature above the cloud point temperature of the polymer.
La présente invention concerne des procédés nouveaux de séparation ou de purification, ou les deux, de compositions biologiques. Ces compositions biologiques comprennent un peptide liant les polysaccharides et elles peuvent synthétisées en totalité ou en partie, ou préparées grâce à la technique de l'ADN recombinant. Selon le procédé, une solution contenant la composition biologique est mélangée à un oligosaccharide formant les phases, auquel se lie le peptide liant, et la séparation des phases est provoquée. La phase qui contient la composition biologique est ensuite isolée. On provoque la séparation des phases en utilisant un agent approprié ou, quand le polymère d'oligosaccharide est un polymère à séparation thermique, en portant la température au-dessus du point de trouble de ce polymère.
Haynes Charles A.
Kilburn Douglas G.
Tomme Peter
Mbm & Co.
University Of British Columbia
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