Separation device and separation method for biomolecular...

G - Physics – 01 – N

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G01N 27/447 (2006.01)

Patent

CA 2505255

The invention concerns a device for separating and a method for separating a biomolecular substance sample, in particular protein mixtures. Said device comprises a separating element (10) designed to ensure a two-dimensional separation, preferably electrophoretic of constituents of the substance sample in the region (20) of a separation plane. The invention is characterized in that the separating element (10) has a channel or transfer structure (14) for locally accurate transfer of the separated sample constituents in a transport direction transverse to the separation plane onto a support surface (16) adapted to mass spectroscopy research.

La présente invention concerne un dispositif de séparation et un procédé de séparation pour un échantillon de matière biomoléculaire, notamment des mélanges protéiques. Ce dispositif comprend un élément de séparation (10) conçu pour effectuer une séparation bidimensionnelle de préférence électrophorétique de composants de l'échantillon de matière dans la zone (30) d'un plan de séparation. Selon cette invention, l'élément de séparation (10) présente une structure de canal ou de transfert (14) permettant de transférer de façon localement précise des composants d'échantillon séparés dans une direction de transport transversale au plan de séparation sur une surface de support (16) adaptée à des recherches par spectroscopie de masse.

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