Separation-free assay method

G - Physics – 01 – N

Patent

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Details

G01N 33/58 (2006.01) G01N 33/536 (2006.01) G01N 33/542 (2006.01)

Patent

CA 2641360

This invention relates to a separation-free assay method employing a binding partner label and a nonspecifically binding label, wherein a signal from said binding partner label or said nonspecifically binding label is measured in a binding event, said method comprising the use of a) two or more binding partners, and a directly luminescent binding partner label adsorbed and/or covalently coupled to at least one of said binding partners; and b) said nonspecifically binding label, wherein i) said nonspecifically binding label is able to affect the signal of said binding partner label, or ii) said binding partner label is able to affect the signal of said nonspecifically binding label; and wherein at least one of said binding partners is a mobile binding partner and at least one other of said binding partners is a labelled ligand.

La présente invention porte sur un procédé de dosage sans séparation employant un marqueur partenaire de liaison et un marqueur de liaison non spécifique, un signal de ledit marqueur partenaire de liaison ou de ledit marqueur de liaison non spécifique étant mesuré en cas de liaison, ledit procédé comprenant l'utilisation de a) deux ou plus de deux partenaires de liaison, et d'un marqueur partenaire de liaison directement luminescente adsorbée et/ou couplée de manière covalente à au moins l'un desdits partenaires de liaison ; et b) ledit marqueur de liaison non spécifique, i)ledit marqueur de liaison non spécifique étant capable d'affecter le signal de ledit marqueur partenaire de liaison, ou ii) ledit marqueur partenaire de liaison étant capable d'affecter le signal de ledit marqueur de liaison non spécifique, et au moins l'un desdits partenaires de liaison étant un partenaire de liaison mobile et au moins un autre desdits partenaires de liaison étant un ligant marqué.

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