Separation-free specific binding assays using anti-inhibitor...

Details Separation-free specific binding assays using anti-inhibitor... Separation-free specific binding assays using anti-inhibitor...

G - Physics

01

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G01N 33/566 (2006.01) C12N 9/96 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/577 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2150496

Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, a reporter enzyme, an inhibitor antibody and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti- inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the target specific binding ligand to the signal generated without the use of separation or wash steps.

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