Separation material for blood coagulation factor'...

C - Chemistry – Metallurgy – 12 – N

Patent

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167/114, 195/39,

C12N 9/48 (2006.01) A61K 35/14 (2006.01) B01J 20/32 (2006.01) C07K 1/22 (2006.01) C07K 14/755 (2006.01)

Patent

CA 2032607

ABSTRACT OF THE DISCLOSURE A separation material capable of realizing efficient separation and recovery of a blood coagulation factor, especially blood coagulation factor VIII or complex thereof with von Willebrand factor, from plasma or from samples containing blood coagulation factors. The separation material for separating and recovering a blood coagulation factor comprises a porous matrix having linked thereon one or more ligands each consisting of a radical exhibiting an affinity to the blood coagulation factor to be recovered, said matrix having a specific surface area of at least 1.5 m2 per milliliter of the separation material with respect to pores having diameters of at least 0.1 µ m and being derived from a porous particulate material having an exclusion limit molecular weight of at least 1.5x 106 as determined with polyethylene glycol. The separation material is prepared by the process steps of subjecting a porous particulate material having an exclusion limit molecular weight of at least 1.5x 106 to an activation by 1,1'-carbonydiimidazolating, tresylating, carbodiimidizing, thiopropylating, epoxidizing, bromocyani- zing or formylating using corresponding activating agent and reacting the so activated porous particulate material with one or more substances which exihit after being linked as ligand on the porous particulate material an affinity to the blood coagulation factor to be recovered, so as to form a porous matrix having a specific surface area of at least 1.5 m2 per milliliter of the porous particulate material with respect to pores having diameters of at least 0.1 µm.

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