C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/00 (2006.01) C07K 1/28 (2006.01)
Patent
CA 2162671
The present invention provides a method for the separation of anti-metal chelate antibodies from non-specific proteins, including antibodies, by applying a preparation containing the anti-metal chelate antibodies to a carboxylic acid derivatized solid support and eluting first with an elution buffer containing sufficient salt concentration to elute non-specific proteins but not sufficient to elute the anti-metal chelate antibodies and then increasing the salt concentration of the elution solution so as to elute the anti-metal chelate antibodies. In one embodiment, the carboxylic acid derivatized solid support is a carboxymethyl resin. Appropriate salts include sodium phosphate, sodium chloride, sodium acetate and sodium sulfate. The method can be used to separate monoclonal or polyclonal anti-metal chelate antibodies from non-specific proteins as well as to separate bifunctional anti-metal chelate antibodies from monoclonal anti-metal chelate antibodies and other non-specific proteins. The method is also useful for separating anti-metal chelate antibody fragments bearing antigen reactive regions from non-specific proteins.
Hybritech Incorporated
Sim & Mcburney
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