Separation of anti-metal chelate antibodies

Details Separation of anti-metal chelate antibodies Separation of anti-metal chelate antibodies

C - Chemistry, Metallurgy

07

K

167/139, 195/1.1

C07K 16/00 (2006.01) C07K 1/18 (2006.01) C07K 16/44 (2006.01)

Patent

CA 2022185

ABSTRACT OF THE INVENTION The present invention provides a method for the separation of anti-metal chelate antibodies from non- specific proteins, including antibodies, by applying a preparation containing the anti-metal chelate antibodies to an oxo acid derivatized solid support and eluting first with an elution buffer containing sufficient salt concentration to elute non-specific proteins but not sufficient to elute the anti-metal chelate antibodies and then eluting the anti-metal chelate antibodies.

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