Separation of avian antibodies

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/26 (2006.01) B01D 57/02 (2006.01) C07K 16/02 (2006.01)

Patent

CA 2405694

A method for obtaining avian antibodies from eggs, the method comprising the steps of:- (a) providing egg material containing avian antibodies; (b) adjusting the pH of the egg material such that the antibodies in the sample have a desired charge; (c) placing the pH adjusted egg material in a first solvent stream, the first solvent stream being separated from a second solvent stream by an electrophoretic separation membrane having a molecular mass cut- off greater than the molecular mass of avian antibodies, the second solvent stream comprising a solvent with a desired pH; (d) applying an electric potential between the first and second solvent streams causing avian antibodies to move through the electrophoretic separation membrane into the second solvent stream while other components in the egg material are substantially retained in the first solvent stream, or if entering the electrophoresis separation membrane, being substantially prevented from passing through the electrophoresis separation membrane to the second solvent stream; and (e) maintaining steps (d) until the desired amount of avian antibodies has been obtained from the egg material.

L'invention concerne un procédé permettant d'obtenir des anticorps aviens à partir d'oeufs. Ce procédé consiste a) à fournir un matériau d'oeuf contenant des anticorps aviens; b) à régler le pH du matériau d'oeuf de sorte que les anticorps de l'échantillon possèdent une charge désirée; c) à placer le matériau d'oeuf à pH réglé dans un premier flux de solvant, ce flux étant séparé d'un second flux de solvant au moyen d'une membrane de séparation électrophorétique possédant une séparation de masse moléculaire supérieure à la masse moléculaire des anticorps aviens, le flux du second solvant comprenant un solvant à pH désiré; d) à appliquer un potentiel électrique entre les premier et second flux de solvant, ce qui permet aux anticorps aviens de traverser la membrane de séparation électrophorétique vers le second flux de solvant pendant que les autres composants du matériau d'oeuf sont sensiblement retenus dans le premier flux de solvant, ou ce qui empêche sensiblement lesdits anticorps de traverser la membrane de séparation électrophorétique vers le second flux de solvant dans le cas où ceux-ci entrent dans la membrane de séparation électrophorétique; et e) à renouveler l'étape (d) jusqu'à ce qu'on obtienne la quantité d'anticorps aviens désirée à partir du matériau d'oeuf.

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