Separation of contaminants from streptococcus pneumoniae...

Details Separation of contaminants from streptococcus pneumoniae... Separation of contaminants from streptococcus pneumoniae...

C - Chemistry, Metallurgy

08

B

C08B 37/00 (2006.01) A61K 39/09 (2006.01)

Patent

CA 2604362

A process for reducing the protein content and preserving the capsular polysaccharide content in a complex cellular Streptococcus pneumoniae lysate broth prior to purification is described. Utilizing pH reduction after cellular lysis has resulted in a purified polysaccharide that consistently meets the protein specification, and higher recovery yields of polysaccharide during the purification process.

L'invention concerne un procédé de réduction du contenu en protéines et de conservation du contenu en polysaccharides sous forme de capsule dans un bouillon de lysat cellulaire complexe Streptococcus pneumoniae avant purification. L'utilisation de la réduction en pH après lyse cellulaire permet d'obtenir un polysaccharide purifié qui répond pleinement aux spécifications de la protéine, ainsi que des rendements de récupération de polysaccharide élevés pendant le processus de purification.

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