Separation of human serum albumin

Details Separation of human serum albumin Separation of human serum albumin

C - Chemistry, Metallurgy

07

K

C07K 14/765 (2006.01)

Patent

CA 2195202

Milk from transgenic bovine animals contains human serum albumin (hSA) and endogenous bovine serum albumin (bSA). The hSA is purified to at least 98 % purity by a separation method which includes affinity chromatography in the presence of a detergent; the affinity ligand being triazine dye molecules. The affinity chromatography step can be incorporated into a protocol to provide essentially pure recombinant hSA as determined by SDS-PAGE and radioimmunoassays.

Du lait provenant de bovins transgéniques contient de l'albumine sérique humaine (hSA) et une albumine sérique bovine endogène (bSA). On purifie la hSA jusqu'à une pureté d'au moins 98 % par un procédé qui inclut une chromatographie d'affinité en présence d'un détergent, le ligand d'affinité étant des molécules d'un colorant, la triazine. L'étape de chromatographique d'affinité peut être incorporée dans une protocole visant à fournir une hSA recombinée pure par SDS-PAGE et des radio-immunodosages.

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