Separation of saccharides by exclusion chromatography

Details Separation of saccharides by exclusion chromatography Separation of saccharides by exclusion chromatography

C - Chemistry, Metallurgy

07

H

260/220.1, 260/2

C07H 1/06 (2006.01) B01D 15/08 (2006.01) C13K 13/00 (2006.01)

Patent

CA 1065313

ABSTRACT OF TEE DISCLOSURE A process for separating saccharides by exclusion chromatography wherein a feed mixture of saccharides is supplied to a molecular exclusion chromatography zone from which at least three fractions are removed. The first fraction contains the least diffusible saccharides of the mixture which generally are the highest molecular weight components, the second fraction contains predominantly intermediate molecular weight saccharides and the third fraction contains predominantly lower molecular weight saccharide fractions. After supplying the feed to the column, the zone is continuously eluted, first with the first fraction removed from the column followed by elution with water. The use of the first fraction from the column as an eluting solvent serves to minimize the amount of water employed as an eluting solvent.

280131

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