Sequence based engineering and optimization of single chain...

C - Chemistry – Metallurgy – 12 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 15/00 (2006.01) A61K 39/395 (2006.01) C07K 16/00 (2006.01) G01N 33/50 (2006.01) C12N 15/13 (2006.01)

Patent

CA 2689064

The invention provides methods of using sequence based analysis and rational strategies to modify and improve the structural and biophysical properties of single chain antibodies (scFvs), including stability, solubility, and antigen binding affinity. These methods and strategies can be used individually or in combination. The methods of the present invention also include the use of a database comprising scFv sequences from an experimentally screened scFv library of antibodies that have been selected to have superior solubility and stability. The invention also provides methods of using the properties found for these selected antibodies in a general approach for reshaping scFv antibodies to improve stability and solubility properties of a single chain antibody fragment.

L'invention concerne des méthodes consistant à utiliser une analyse basée sur les séquences et des stratégies rationnelles pour modifier et améliorer les propriétés structurales et biophysiques d'anticorps monocaténaires (scFv), telles que la stabilité, la solubilité et l'affinité de liaison aux antigènes. Ces méthodes et ces stratégies peuvent être utilisées individuellement ou en combinaison. Les méthodes de la présente invention incluent également l'utilisation d'une base de données comprenant des séquences de scFv provenant d'une bibliothèque d'anticorps scFv criblée expérimentalement, les anticorps ayant été sélectionnés pour leur supériorité en termes de solubilité et de stabilité. L'invention concerne également des méthodes d'utilisation des propriétés découvertes pour ces anticorps sélectionnés dans une technique générale de remodelage d'anticorps scFv en vue d'une amélioration des propriétés de stabilité et de solubilité d'un fragment d'anticorps monocaténaire.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Sequence based engineering and optimization of single chain... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sequence based engineering and optimization of single chain..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sequence based engineering and optimization of single chain... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1490695

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.