Sequence capable of accelerating gene expression at...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) C12N 5/10 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2558269

It is intended to increase the protein produced by the recombinant DNA technique with the use of mammalian cells. Transcription can be accelerated by culturing at moderately low temperature with the use of, as an enhancer, a moderately low temperature transcription control element comprising the nucleotide sequence: x1ccccx6x7x8 (wherein x1 represents t, g or a; x6, g, a or c; x7, c, a or t; and x8, c, a or t) or at least one nucleotide sequence complementary to this sequence. Further, translation can be accelerated by culturing at moderately low temperature with the moderately low temperature translation control element of SEQ ID NO: 6 disposed at 3~-side of promoter at 5~-side of the code sequence.

On vise à augmenter la protéine produite par la technique de l~ADN recombinant en utilisant des cellules mammifères. La transcription peut être accélérée par culture à une température modérément faible en utilisant, en tant qu'amplificateur, un élément de contrôle de la transcription à une température modérément faible comprenant la séquence nucléotidique : x¿1?ccccx¿6?x¿7?x¿8? (dans laquelle x¿1? représente t, g ou a ; x¿6?, g, a ou c ; x¿7?, c, a ou t ; et x¿8?, c, a ou t) ou au moins une sequence nucléotidique complémentaire à cette séquence. En outre, la traduction peut être accélérée par culture à une température modérément faible avec un élément de contrôle de la traduction à température modérément faible de SEQ ID NO : 6 disposée à l~extrémité 3~ du promoteur à l~extrémité 5~ de la séquence codante.

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