C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) A61K 31/70 (2006.01) C07H 21/00 (2006.01) C07K 14/47 (2006.01) C12N 15/10 (2006.01) C12N 15/67 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2152501
The present invention defines a DNA protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein-binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.
Andrews Beth M.
Cantor Charles R.
Edwards Cynthia A.
Fry Kirk E.
Turin Lisa M.
Genelabs Technologies Inc.
Gowling Lafleur Henderson Llp
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