Sequence enabled reassembly (seer) - a novel method for...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2607104

The present invention provides a nucleotide sequence detection system in which a reporter enzyme is split into two halves each half of which is associated with at least one zinc finger domain. Upon DNA binding to the specific sequence defined by the zinc finger domains associated with the respective halves, the split-protein reassembles to reconstitute a functional enzyme. As such, the present invention provides methods of using the nucleotide sequence detection system for various diagnostic and identification purposes.

La présente invention concerne un système de détection de séquences nucléotidiques dans lequel une enzyme rapporteur est clivée en deux moitiés, chaque moitié étant associée à au moins un domaine en doigt de zinc. Lors de la liaison de l'ADN à la séquence spécifique définie par les domaines en doigt de zinc associés aux moitiés respectives, la protéine clivée se réassemble afin de reconstituer une enzyme fonctionnelle. Ainsi, la présente invention concerne des méthodes d'utilisation du système de détection de séquences nucléotidiques à diverses fins de diagnostic et d'identification.

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