C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2235327
The present invention is directed to methods for resisting or promoting template independent nucleotide addition to the 3' terminus of a DNA duplex. The process comprises amplifying a target nucleic acid using primers which comprise a 5' terminal sequence which resists or promotes non-templated nucleotide addition to the 3' terminus of the complementary nucleic acid strand. The invention is also directed to a kit for cloning 3' nucleotidylated duplex DNA.
La présente invention se rapporte à des procédés permettant de résister à ou de favoriser l'addition nucléotidique non matricée au niveau de l'extrémité 3' d'une double hélice d'ADN. Ledit procédé consiste à amplifier un acide nucléique cible au moyen d'amorces qui comportent une séquence terminale 5' qui résiste à ou favorise l'addition nucléotidique non matricée au niveau de l'extrémité 3' du brin d'acide nucléique complémentaire. L'invention se rapporte également à un kit conçu pour le clonage d'ADN double hélice portant un groupe nucléotidyle en 3'.
Brownstein Michael J.
Carpten John D.
Smith Jeffrey R.
Department Of Health And Human Services The Government Of The United States Of America Represented By Th
Sim & Mcburney
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