Sequence-specific detection of nucleic acids

Details Sequence-specific detection of nucleic acids Sequence-specific detection of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2214430

The invention concerns a method of detecting point mutations and polymorphisms in nucleic acids and of sequencing unknown nucleic acids by means of a simple method using arrays. According to the method, nucleic acid analogues are used as sequence discriminators. This method facilitates the operating method, even in complex cases.

L'invention concerne un procédé permettant de détecter des mutations ponctuelles et des polymorphismes dans des acides nucléiques, et de séquencer des acides nucléiques inconnus au moyen d'un procédé simple utilisant des séries. Selon ce procédé, on utilise des analogues d'acides nucléiques sous forme de discrimateurs de séquence, ce qui facilite la technique opératoire même en cas de problèmes complexes.

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